Unique blend of Biomedical Science, Pharmacology & Toxicology and Pharmacy training
I am a driven PhD Candidate in Biomedical Sciences with 6 years of integrated pre-clinical, clinical, and translational research experience, committed to bridging the gap between discovery and patient care. My expertise lies in designing and executing experiments, analyzing complex datasets, and translating scientific discoveries into meaningful applications.
With a strong foundation in biomedical research, I am highly motivated to apply my skills in the biopharma industry or clinical trial sector, where I can contribute to drug development, regulatory processes, and innovative therapeutic solutions. I thrive in collaborative, cross-functional environments and am eager to leverage my scientific expertise to drive impactful advancements in healthcare.
Designing and executing complex pre-clinical and translational research studies with rigorous scientific methodology and reproducible protocols.
Deep expertise in drug development processes, pharmacology, and therapeutic innovation with a focus on translational applications.
Comprehensive experience in clinical trial coordination for Phase II and III trials, regulatory compliance, and bridging laboratory discoveries to patient outcomes.
Interpreting complex scientific data, statistical analysis, and drawing actionable insights for research advancement. Softwares: GraphPad Prism, SPSS and R.
Handled 4 different projects at the same time yielding quality data evident with number of publications.
Proficient in a wide array of laboratory techniques, ensuring high-quality experimental execution and data generation across various research domains.
Expertise in PCR, qPCR, Western Blot, ELISA, and DNA/RNA extraction for genetic and protein analysis.
Seahorse analysis for mitochondrial functional screening.
Experienced in advanced methods such as LC-MS, AAV, CRISPR-Cas9, Gene editing and transcription.
Skilled in mammalian cell lines and primary cell cultures, maintaining sterile conditions for diverse experimental setups.
Experienced in the isolation, propagation, and identification of diverse bacterial strains. Established a method for bacterial-extracellular vesicle isolation.
Proficient in the 3D-culture and maintenance of human-derived gastrointestinal and brain organoids.
Proficient in fluorescence and confocal microscopy for detailed cellular and tissue imaging and analysis.
Experienced with rodent models (C57BL/6, db/db, ob/ob, hypertensive), various drug administration routes (IP, IV, SC, PO), meticulous tissue collection and Middle Cerebral Artery Occlusion survival surgery (MCAO, ishemic stroke mice model).
Skilled in multi-color flow cytometry for cellular characterization, including immunophenotyping and cell cycle analysis.
Expert in characterizing extracellular vesicles and synthetic nanoparticles using Nanoparticle Tracking Analysis.
Comprehensive skills in tissue processing, sectioning, staining, and immunohistochemistry for pathological assessment.
Thesis: Role of Adipocyte-derived exosomes in cerebrovascular complications of diabetes
Unravelling the mechanistic involvement of hyperglycemic adipocyte-derived exosomes in causing detrimental effects in the neurovascular unit, including human primary brain microvascular endothelial cells, neurons, astrocytes, and microglial cells.
Studying the effect of diabetic adipocyte-derived exosomes on the sensory motor and cognitive functioning of the brain of mice to understand real-world neurological impacts.
Determining the role of adipocyte-derived exosomes in ischemic stroke paired with diabetic conditions to identify therapeutic targets.
Screening the cargo contents, such as proteins, mRNAs, miRNAs, lipopolysaccharides, and other bioactive molecules of adipocyte-derived exosomes in normal versus diabetic conditions.
Integrated research experience across pre-clinical, clinical, and translational domains
In the field of neurovascular and metabolic diseases, extracellular vesicles
Led major research project through experimental design and management
Standardized protocols implemented with 75% reduced assay variability
Trained and mentored on complex instrumentation and analytical techniques
Achieved in complex MCAO surgery procedures for stroke research
Reduction in processing time through technical optimization projects
Throughout my research career, I have consistently delivered measurable improvements in laboratory efficiency, research quality, and team capability. My work has resulted in significant cost savings, accelerated timelines, and enhanced reproducibility of experimental results.
By establishing fully operational research facilities and implementing rigorous quality control measures, I have created sustainable frameworks that enable cutting-edge biomedical research. My research contributions are to the fields of extracellular vesicles, cerebrovascular and metabolic diseases. My optimization of exosome extraction and purification protocols increased yield by 50%, directly impacting the feasibility and cost-effectiveness of therapeutic development.
These achievements reflect my commitment to excellence in scientific research and my ability to drive meaningful improvements in complex research environments.
Marshall University | July 2022 - May 2026
Leading doctoral research on diabetic adipocyte-derived exosomes in cerebrovascular complications of diabetes, investigating mechanistic pathways in the neurovascular unit and cognitive function.
Marshall University | January 2021 - July 2022
Established fully operational research facility, led $1.5M project on application of Endothelial Progenitor cell-derived extracellular vesicles in ischemic stroke, trained 10+ researchers, and achieved 90% success rate in Middle Cerebral Artery Occlusion survival surgery in mice.
Wright State University | October 2020 - December 2020
Led $50K research project on profiling inflammatory extracellular vesicles in intracerebral hemorrhage patients, and molecular investigations on miR-210 primed endothelial progenitor cell-derived exosomes in ischemic stroke. Contributed to 3 peer-reviewed publications.
Wright State University | January 2019 - March 2020
Investigated Angiotensin II Type 1A Receptor effects in renovascular hypertension model, managed laboratory operations with 100% protocol compliance, presented at 3 international conferences.
Current doctoral research examining how diabetic adipocyte-derived exosomes impact neurovascular function, cognitive performance, and cerebrovascular complications through comprehensive cargo profiling and functional studies.
Studying the therapeutic potential of probiotic strains (Lactobacillus acidophilus and E. coli Nissle 1917) derived extracellular vesicles in cerebrovascular complications in diabetes.
Screening the exosomal miRNA cargo contents of endometriosis patient’s serum, tissue and peritoneal fluid samples.
Evaluating the effects of exosomes extracted from an endometriosis patient (serum, tissue and peritoneal fluid) on the brain in vitro.
Investigating the mechanisms of impaired creatine transport in inflammation.
Exploring creatine supplementation as a long-term adjuvant therapy for inflammatory bowel disease management.
My educational journey reflects a progressive deepening of expertise from pharmaceutical sciences through pharmacology and toxicology to advanced biomedical research, creating a comprehensive foundation for translational research.
- Doctor of Philosophy, Biomedical Sciences - Marshall University (July 2022 - May 2026)
- Master's degree, Pharmacology and Toxicology - Wright State University (August 2018 - May 2020)
- Bachelor's degree, Pharmacy - Savitribai Phule Pune University (July 2014 - July 2018)
- Good Laboratory Practice (GLP)Clinical Research - CITI
- Coordinator Certification (CRC) - CITI
- Indian Pharmacy License - Pharmacy Council of India
- Aseptic Animal Survival Surgery - CITI
- Working With Animals In Biomedical Research - CITI
- Medical Research - CITI
- Toxicology in Drug Discovery and Development - Rutgers
- GCP for Clinical Trials with Investigational Drugs and Medical Devices (U.S. FDA Focus) - CITI
- Six Sigma Green Belt
Oral presentation supported by travel award (Biomolecules) and Early Carer Investigator VIP mentoring session.
$500 cash price for Oral Presentation at 'Research Day 2025', Marshall University.
Digital moderated poster presentation supported by departmental travel award.
Oral Presentation at Marshall University Creative Symposium 2024, representing department of Biomedical Sciences for it's research and excellence.
The miR-210 Primed Endothelial Progenitor Cell Exosomes Alleviate Acute Ischemic Brain Injury - Extensive research article demonstrating therapeutic potential of bioengineered exosomes in stroke treatment.
The profile of inflammatory extracellular vesicles in intracerebral hemorrhage patients - Clinical research article presenting predictive relationships between patient outcomes for intracerebral hemorrhage patients and extracellular vesicles.
A Simplified Method for the Isolation of Extracellular Vesicles - Methodological advancement improving efficiency and reproducibility of exosome research protocols.
Neurovascular Impairment in Type 2 Diabetes Mellitus: The Role of Adipocyte-Derived Exosomes - Co-corresponding authored review article discussing current knowledge and gaps of Adipocyte-derived exosomes in T2DM-associated cerebrovascular complications.
For a comprehensive list of publications and full access to research, please visit my NCBI My Bibliography profile.
I am actively seeking opportunities in the biopharma industry or clinical research organizations where I can apply my extensive research expertise to drive drug development, regulatory processes, and innovative therapeutic solutions. My unique combination of pharmaceutical knowledge, translational research experience, and proven track record in project management positions me to make immediate contributions to your organization.
Whether you're looking for expertise in extracellular vesicle research, neurovascular disorders, metabolic diseases, or clinical trial coordination, I bring a collaborative mindset and a passion for translating scientific discoveries into meaningful patient outcomes.
I thrive in cross-functional environments and am eager to leverage my skills in experimental design, data analysis, and scientific communication to advance healthcare innovation. Let's discuss how my background aligns with your organization's goals.
Location: Huntington, West Virginia, United States 25701
Email: [email protected] or [email protected]
- Extracellular Vesicles Research
- Neurovascular Disorders
- Metabolic Diseases
- Translational Research
- Clinical Trial Coordination
- Drug Development
Translational Research on Extracellular Vesicles, Metabolic Diseases & Neurovascular Disorders | Pharmacist Scientist | Aspiring Post-doc Scientist in Academia/Biopharma/CRO